Cloning and expression of human and rat Dt dopamine receptors

@article{Zhou1990CloningAE,
  title={Cloning and expression of human and rat Dt dopamine receptors},
  author={Qun Zhou and D. Grandy and L. Thambi and J. Kushner and H. V. Tol and R. Cone and D. Pribnow and J. Salon and J. Bunzow and O. Civelli},
  journal={Nature},
  year={1990},
  volume={347},
  pages={76-80}
}
THE importance of the dopaminergic system in brain function has been emphasized by its association with neurological and psychiatric disorders such as Parkinson's disease and schizophrenia. On the basis of their biochemical and pharmacological characteristics, dopamine receptors are classified into D1 and D2 subtypes1,2. As the most abundant dopamine receptor in the central nervous system, D1 receptors seem to mediate some behavioural responses3, modulate activity of D2 dopamine receptors4,5… Expand
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This receptor has been characterized on the basis of three criteria: the deduced amino-acid sequence which reveals that it is a member of the family of G-protein-coupled receptors; the tissue distribution of the mRNA which parallels that of the D2 dopamine receptor; and the pharmacological profile of mouse fibroblast cells transfected with the cDNA. Expand
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The present review focuses on the hypothesized D1/D2 dopamine (DA) receptor classification, originally based on the form of receptor coupling to adenylate cyclase activity. The pharmacologicalExpand
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A clone encoding a human D2 dopamine receptor was isolated from a pituitary cDNA library and sequenced, and it was shown that the coding sequence is interrupted by six introns and that the additional amino acids present in the human pituitsary receptor are encoded by a single exon of 87 base pairs. Expand
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Competition curve analysis with a variety of adrenergic ligands suggests that this cDNA clone represents the alpha 2B-adrenergic receptor, which is consistent with the structure of other members of the family of guanine nucleotide-binding protein-coupled receptors. Expand
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RNA blot analysis indicates a message of 2.5 kilobases in rat tissues, with a pattern of tissue distribution consistent with beta 1AR binding, which suggests that the avian gene encoding beta AR and the human gene encodingbeta 1AR evolved from a common ancestral gene. Expand
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The distribution of D1 and D2 receptors was studied in coronal sections of rat brain, using quantitative autoradiography. D1 receptors were labeled with 1.8 nM 3H-SKF-83566 (a brominated analog ofExpand
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Novel beta-AR agonists having high thermogenic, antiobesity, and antidiabetic activities in animal models are among the most potent stimulators of the beta 3-AR. Expand
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The gene for the human platelet alpha 2-adrenergic receptor has been cloned with oligonucleotides corresponding to the partial amino acid sequence of the purified receptor, and two related genes have been identified by low stringency Southern blot analysis. Expand
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