Cloning and expression of human and rat Dt dopamine receptors

  title={Cloning and expression of human and rat Dt dopamine receptors},
  author={Qun Yong Zhou and David K. Grandy and Lisa Thambi and Jake A. Kushner and Hubert H.M. Van Tol and Roger D. Cone and David Pribnow and John A. Salon and James R. Bunzow and Olivier Civelli},
THE importance of the dopaminergic system in brain function has been emphasized by its association with neurological and psychiatric disorders such as Parkinson's disease and schizophrenia. On the basis of their biochemical and pharmacological characteristics, dopamine receptors are classified into D1 and D2 subtypes1,2. As the most abundant dopamine receptor in the central nervous system, D1 receptors seem to mediate some behavioural responses3, modulate activity of D2 dopamine receptors4,5… 

Cloning of the gene for a human dopamine D5 receptor with higher affinity for dopamine than D1

The cloning of a gene encoding a 477-amino-acid protein with strong homology to the cloned Dt receptor is reported here the existence of a dopamine D1-like receptor with these characteristics had not been predicted and may represent an alternative pathway for dopamine-mediated events and regulation of D2 receptor activity.

Cloning of the gene for a human dopamine D4 receptor with high affinity for the antipsychotic clozapine

The cloning of a gene that encodes a dopamine receptor gene that has high homology to the human dopamine D2 and D3 receptor genes is reported, which suggests the existence of other types of dopamine receptors which are more sensitive to clozapine.

Expression of the dopamine D2 receptor gene in brain

Brain Dopamine Receptors: A Primer on Their Current Status, Basic and Clinical

The discovery and definition of new gene products now require long‐term efforts to clarify their physiology and to develop a pharmacology of novel dopamine receptors and their effectors.

Subtypes and localization of dopamine receptors in human brain

  • J. Keyser
  • Biology, Psychology
    Neurochemistry International
  • 1993

The Dopamine D1 Receptors

Dopaminergic receptors are members of a large gene family of hormone/neurotransmitter receptors that exert their biological actions via signal transduction pathways that involve guanine nucleotide-binding proteins.

The dopamine D(4) receptor: one decade of research.

Dopamine receptors and brain function




Cloning and expression of a rat D2 dopamine receptor cDNA

This receptor has been characterized on the basis of three criteria: the deduced amino-acid sequence which reveals that it is a member of the family of G-protein-coupled receptors; the tissue distribution of the mRNA which parallels that of the D2 dopamine receptor; and the pharmacological profile of mouse fibroblast cells transfected with the cDNA.

The classification of dopamine receptors: relationship to radioligand binding.

Since 1975, the elegantly simple radioligand binding technique has direct examination of the interactions of agonists and antagonists with putative dopamine receptors (DARs), and these studies form the major focus of this review.

Review: D1 dopamine receptor—the search for a function: A critical evaluation of the D1/D2 dopamine receptor classification and its functional implications

A variety of biochemical, behavioral, and electrophysiological evidence is presented which supports the view that D1 and D2 DA receptors can interact in both an opposing and synergistic fashion.

Cloning of the cDNA and gene for a human D2 dopamine receptor.

A clone encoding a human D2 dopamine receptor was isolated from a pituitary cDNA library and sequenced, and it was shown that the coding sequence is interrupted by six introns and that the additional amino acids present in the human pituitsary receptor are encoded by a single exon of 87 base pairs.

D1 dopamine receptor activation required for postsynaptic expression of D2 agonist effects.

The previously held view that D2 receptors alone are responsible for evoking the changes in behavior and basal ganglia output induced by nonselective dopamine agonists and endogenous dopamine is not supported by these results, which indicate that these phenomena require concurrent stimulation of both dopamine receptor subtypes.

Cloning and expression of a human kidney cDNA for an alpha 2-adrenergic receptor subtype.

Competition curve analysis with a variety of adrenergic ligands suggests that this cDNA clone represents the alpha 2B-adrenergic receptor, which is consistent with the structure of other members of the family of guanine nucleotide-binding protein-coupled receptors.

Cloning of the cDNA for the human beta 1-adrenergic receptor.

RNA blot analysis indicates a message of 2.5 kilobases in rat tissues, with a pattern of tissue distribution consistent with beta 1AR binding, which suggests that the avian gene encoding beta AR and the human gene encodingbeta 1AR evolved from a common ancestral gene.

Quantitative autoradiographic localization of the D1 and D2 subtypes of dopamine receptors in rat brain

The distribution of D1 and D2 receptors was studied in coronal sections of rat brain, using quantitative autoradiography. D1 receptors were labeled with 1.8 nM 3H-SKF-83566 (a brominated analog of

Molecular characterization of the human beta 3-adrenergic receptor.

Novel beta-AR agonists having high thermogenic, antiobesity, and antidiabetic activities in animal models are among the most potent stimulators of the beta 3-AR.

Cloning, sequencing, and expression of the gene coding for the human platelet alpha 2-adrenergic receptor.

The gene for the human platelet alpha 2-adrenergic receptor has been cloned with oligonucleotides corresponding to the partial amino acid sequence of the purified receptor, and two related genes have been identified by low stringency Southern blot analysis.