Cloning and expression of herpes simplex virus type 1 glycoprotein L gene

@inproceedings{Qihan2007CloningAE,
  title={Cloning and expression of herpes simplex virus type 1 glycoprotein L gene},
  author={Li Qi-han},
  year={2007}
}
The herpes simplex virus type 1(HSV-1) glycoprotein L(gL) gene was amplified by PCR and cloned into the prokaryotic expressive vector pGEX-5X-1.The recombinant vector pGEX-5X-1-gL was identified by PCR,restriction digestion and sequence analysis.GST-gL fusion protein was expressed efficiently in E.coli BL21 though IPTG as an inducer.The fusion protein band with size of 56?ku was detected by SDS-PAGE.An optimal expression system was established though screen IPTG concentration and inducing… CONTINUE READING

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