• Medicine
  • Published in
    European journal of…
    1989

Cloning and expression of gamma-glutamyl transpeptidase from isolated porcine brain capillaries.

@article{Papandrikopoulou1989CloningAE,
  title={Cloning and expression of gamma-glutamyl transpeptidase from isolated porcine brain capillaries.},
  author={Anastasia Papandrikopoulou and Andreas Frey and Hans G{\"u}nter Gassen},
  journal={European journal of biochemistry},
  year={1989},
  volume={183 3},
  pages={
          693-8
        }
}
In order to understand the mechanisms regulating the blood-brain barrier we isolated a cDNA clone for gamma-glutamyl transpeptidase from purified porcine brain capillaries by cross-species DNA hybridization using a DNA fragment from a rat kidney cDNA clone. The sequence of the porcine gamma-glutamyl transpeptidase cDNA shows 84% identity in the coding region to the sequence of human placenta gamma-glutamyl transpeptidase cDNA. The derived protein sequences of the human and porcine gamma… CONTINUE READING