Cloning and expression of earthworm fibrinolytic enzyme PM(246) in Pichia pastoris.

@article{Hu2005CloningAE,
  title={Cloning and expression of earthworm fibrinolytic enzyme PM(246) in Pichia pastoris.},
  author={Yan Feng Hu and Xiao-lin Meng and Jin-ping Xu and Wei Ming Lu and Jian Wang},
  journal={Protein expression and purification},
  year={2005},
  volume={43 1},
  pages={18-25}
}
We have cloned, expressed, and purified a novel earthworm fibrinolytic enzyme (EFE) of Lumbricus rubellus in Pichia pastoris. Its cDNA sequence revealed a 747bp region containing an intact ORF that encodes a protein of 246 amino acid residues, designated as EFE PM(246). While EFE PM(246) is distinct, its cDNA shows a high degree of sequence homologies with… CONTINUE READING