Cloning and expression of distinct nitrite reductases in tobacco leaves and roots

@article{Kronenberger1993CloningAE,
  title={Cloning and expression of distinct nitrite reductases in tobacco leaves and roots},
  author={Jocelyne Kronenberger and Andr{\'e}e L{\'e}pingle and Michel Caboche and Herv{\'e} Vaucheret},
  journal={Molecular and General Genetics MGG},
  year={1993},
  volume={236},
  pages={203-208}
}
Three tobacco nitrite reductase (NiR) cDNA clones were isolated using spinach NiR cDNA as a probe. Sequence analysis and Southern blot hybridization revealed four genes in tobacco. Two of these genes presumably derived from the ancestral species Nicotiana tomentosiformis, the other two from the ancestor N. sylvestris. Northern blot analysis showed that one gene from each ancestral genome was expressed predominantly in leaves, whilst RNA from the other was detected mostly in roots. The… CONTINUE READING