Cloning and expression of cytosolic phosphoglycerate kinase from pea (Pisum sativum L.).

Abstract

In common with several other respiratory and photosynthetic enzymes, a sub-population of cytosolic phosphoglycerate kinase (PGK) occurs in the nucleus in pea leaves and shoots. The full-length cDNA encoding pea cytosolic PGK has been cloned and sequenced, revealing not only the PGK 'signature' but also a nuclear localization signal (NLS). A translational fusion of PGK and GFP was used to transform tobacco BY-2 cells resulting in GFP locating to the cell nuclei.

Cite this paper

@article{Brice2004CloningAE, title={Cloning and expression of cytosolic phosphoglycerate kinase from pea (Pisum sativum L.).}, author={David C Brice and John A . Bryant and Gerardas Dambrauskas and Suzanne Drury and Jennifer A. Littlechild}, journal={Journal of experimental botany}, year={2004}, volume={55 398}, pages={955-6} }