Cloning and expression of cDNA encoding human basic fibroblast growth factor

  title={Cloning and expression of cDNA encoding human basic fibroblast growth factor},
  author={Tsutomu Kurokawa and Reiko Sasada and Makoto Iwane and Koichi Igarashi},
  journal={FEBS Letters},
Differential expression of mRNA coding for heparin‐binding growth factor type 2 in human cells
The results suggest that heparin‐binding growth factor type 2/basic fibroblast growth factor (HBGF‐2/bFGF) may mediate the proliferation of epidermal cells through paracrine mechanisms involving stromal fibroblasts.
Molecular cloning of a novel cytokine cDNA encoding the ninth member of the fibroblast growth factor family, which has a unique secretion property
Restricted gene expression in organs and the unique secretion nature of the protein suggest that FGF-9 plays a physiological role which differs from those of well-characterized acidic FGF and basic FGF.
Transformation of mouse BALB/c 3T3 cells with human basic fibroblast growth factor cDNA
The expression of human basic fibroblast growth factor cDNA in mouse BALB/c 3T3 clone A31 cells induced morphological transformation and the results suggest that the cellular transformation mediated by bFGF is caused by autocrine stimulation with secreted bF GF molecules.
The expression of basic fibroblast growth factor and its receptor in cell lines derived from normal human mammary gland and a benign mammary lesion.
The results indicate that differentiation to the human myoepithelial-like phenotype in culture is associated with the enhanced expression of bFGF, and it is suggested that bF GF, immunocytochemically detected in the basement membrane of the human breast, may arise, at least in part, from the myoEPithelial cells of the mammary parenchyma.
Detection of 28S RNA with the FGF-2 cDNA at high stringency through related G/C-rich sequences
Data indicate that the 4.7 kb RNA detected in total rat glial tumor cell RNA is not a bona fide FGF-2 transcript, and most likely represents cross hybridization with abundant 28S RNA through G/C-rich non-coding sequences present in the ‘intact’ rat F GF-2 cDNA.
Messenger RNA stabilization accounts for elevated basic fibroblast growth factor transcript levels in a human astrocytoma cell line.
Examination of the half-life of bFGF mRNA in two human tumor cell lines shows that posttranscriptional processes play an important role in the regulation of b FGF transcript levels and demonstrates that loss of post transcriptional regulation could contribute to elevated bF GF expression in some tumors.


Human basic fibroblast growth factor: nucleotide sequence and genomic organization.
Southern blot analysis of human genomic DNA and mapping of the cloned gene shows that there is only one basic FGF gene, and all of the basic, heparin‐binding endothelial cell mitogens of similar amino acid composition that have been described must be products of this single gene.
Nucleotide sequence of a bovine clone encoding the angiogenic protein, basic fibroblast growth factor.
An oligonucleotide probe was designed from the nucleotide sequence of the amino-terminal exon of bovine acidic FGF, taking into account the 55 percent amino acid sequence homology between the two factors.
Nucleotide sequence of human monocyte interleukin 1 precursor cDNA.
The cDNA nucleotide sequence suggests that IL-1 is initially translated as a precursor molecule that is subsequently processed into the 15,000-20,000 Mr protein usually associated with IL- 1 activity.
A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells.
A plasmid vector for cloning cDNAs in Escherichia coli is described; the same vector also promotes expression of the cDNA segment in mammalian cells, and it is confirmed that the alpha-globin transcript produced in transfected cells is initiated correctly, spliced at either of the two introns, and polyadenylated either at the site coded in the c DNA segment or at the distal SV40polyadenylation signal.
Primary structure of bovine pituitary basic fibroblast growth factor (FGF) and comparison with the amino-terminal sequence of bovine brain acidic FGF.
  • F. EschA. Baird R. Guillemin
  • Biology, Chemistry
    Proceedings of the National Academy of Sciences of the United States of America
  • 1985
The two major mitogenic polypeptides for endothelial cells have been purified to homogeneity and the available protein sequence data demonstrate the existence of significant structural homology between the two polyPEptides.
An endothelial cell growth factor from bovine hypothalamus: identification and partial characterization.
Neither the biologically active FGF-like mitogen purified from the hypothalamus extracts nor FGF purified from bovine pituitary glands was mitogenic when added to human endothelial cells in vitro, suggesting the presence of more than one mitogen in the hypothalamic extracts.
3′ Non-coding region sequences in eukaryotic messenger RNA
A large selection of the 3′ non-coding regions of rabbit and human globin mRN As are 85% homologous, demonstrating that this region is significantly conserved in evolution.
Angiogenic activity of human tumor plasma membrane components.
Plasma membranes from the human colon adenocarcinoma cell line HT-29 have been isolated and examined for the presence of angiogenic activity. Membrane-associated macromolecules extracted with Triton