Cloning and expression of cDNA coding for bouganin.

@article{Hartog2002CloningAE,
  title={Cloning and expression of cDNA coding for bouganin.},
  author={Marcel T den Hartog and Chiara Lubelli and Louis Boon and Sijmie Heerkens and Antonio P Ortiz Buijsse and Mark de Boer and Fiorenzo Stirpe},
  journal={European journal of biochemistry},
  year={2002},
  volume={269 6},
  pages={1772-9}
}
Bouganin is a ribosome-inactivating protein that recently was isolated from Bougainvillea spectabilis Willd. In this work, the cloning and expression of the cDNA encoding for bouganin is described. From the cDNA, the amino-acid sequence was deduced, which correlated with the primary sequence data obtained by amino-acid sequencing on the native protein. Bouganin is synthesized as a pro-peptide consisting of 305 amino acids, the first 26 of which act as a leader signal while the 29 C-terminal… CONTINUE READING