Cloning and expression of an intracellular alkaline protease gene from alkalophilic Thermoactinomyces sp. HS682.

@article{Tsuchiya1997CloningAE,
  title={Cloning and expression of an intracellular alkaline protease gene from alkalophilic Thermoactinomyces sp. HS682.},
  author={Kayoko S Tsuchiya and Ikuo Ikeda and Tomohiro Tsuchiya and Tetsuya Kimura},
  journal={Bioscience, biotechnology, and biochemistry},
  year={1997},
  volume={61 2},
  pages={298-303}
}
An intracellular alkaline serine protease gene of alkalophilic Thermoactinomyces sp. HS682 was cloned and expressed in Escherichia coli. Sequence analysis showed a putative promoter region, a putative transcriptional termination signal, and an open reading frame of 963 bases, coding for a polypeptide of 321 amino acids. The protease expressed in E. coli was purified by DEAE-Toyopearl 650M and Sephadex G-75 chromatography. The N-terminal sequence (30 amino acids) of the purified protein was… CONTINUE READING