Cloning and expression of an autoimmune DNA-binding single chain Fv. Only the heavy chain is required for binding.

Abstract

Hed 10 is a murine autoimmune antibody which binds tightly to the single-stranded DNA, poly(dT). The heavy and light chain variable region genes of Hed 10 were cloned and then joined by a 42 base-pair linker with the aid of a PCR ligation technique to produce a single chain Fv gene. After insertion into an expression vector the single chain Fv protein (scFv… (More)

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