Cloning and expression of an actinokinase gene from a thermophilic Streptomyces in Escherechia coli.

@article{Chitte2001CloningAE,
  title={Cloning and expression of an actinokinase gene from a thermophilic Streptomyces in Escherechia coli.},
  author={R R Chitte and Satyahari Dey},
  journal={Indian journal of experimental biology},
  year={2001},
  volume={39 5},
  pages={410-5}
}
A thermophilic Streptomyces megasporus strain SD5, could secrete a new fibrinolytic (actinokinase) at 55 degrees C. The gene (ackS) encoding actinokinase was isolated from the chromosomal DNA of S. megasporus SD5 and cloned in different hosts and vectors. The expression was obtained in E. coli JM109 using Cla I linearized pBR322 as vector (pSR 500). The… CONTINUE READING