Cloning and expression of a thermophilic α-amylase gene from Bacillus stearothermophilus in Escherichia coli

@article{Tsukagoshi1984CloningAE,
  title={Cloning and expression of a thermophilic α-amylase gene from Bacillus stearothermophilus in Escherichia coli},
  author={Norihiro Tsukagoshi and Hideshi Ihara and Hiroshi Yamagata and Shigezo Udaka},
  journal={Molecular and General Genetics MGG},
  year={1984},
  volume={193},
  pages={58-63}
}
A 6.4 Kb HindIII fragment of Bacillus stearothermophilus DY-5 DNA cloned in Escherichia coli using pBR322 as a vector was shown to direct the synthesis of a thermophilic α-amylase. In attempts to reduce the size of the insert, the α-amylase gene was shown to be contained in a 3.1 Kb HindIII-BamHI fragment of the donor strain DNA. The α-amylase gene was stably maintained and expressed efficiently in E. coli. The enzymic properties of α-amylase produced in E. coli closely resembled those of the… CONTINUE READING
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