Cloning and expression of a collagen-analog-encoding synthetic gene in Escherichia coli.

@article{Goldberg1989CloningAE,
  title={Cloning and expression of a collagen-analog-encoding synthetic gene in Escherichia coli.},
  author={Ina Goldberg and Anthony J. Salerno and Tricia Patterson and Jon I. Williams},
  journal={Gene},
  year={1989},
  volume={80 2},
  pages={305-14}
}
A family of totally synthetic genes coding for multiple tandem repeats of the amino acid sequence (Gly-Pro-Pro) has been prepared and inserted into the ClaI cloning site of the expression vector pJL6. A representative recombinant plasmid, pAC1, with an insert of about 340 bp was established in an Escherichia coli strain bearing a defective lambda prophage… CONTINUE READING