Cloning and expression of a seed-specific metallothionein-like protein from sesame.

  title={Cloning and expression of a seed-specific metallothionein-like protein from sesame.},
  author={Chia-lin Chyan and Tiger T T Lee and Chiung-Pin Liu and Yuan-Chang Yang and Jason T. C. Tzen and Wing-Ming Chou},
  journal={Bioscience, biotechnology, and biochemistry},
  volume={69 12},
A cDNA clone, SiMT encoding an Ec type of metallothionein (MT)-like protein, was isolated from maturing seeds of sesame (Sesamum indicum L.), and its deduced protein sequence shared 47-65% similarity to other known Ec type of MT-like proteins with three highly conserved cysteine-rich segments. The transcript of SiMT was exclusively accumulated in maturing seeds from two weeks after flowering to the end of seed maturation. The results of a southern blot analysis suggested that one SiMT and one… CONTINUE READING
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