Cloning and expression of a rat D2 dopamine receptor cDNA

@article{Bunzow1988CloningAE,
  title={Cloning and expression of a rat D2 dopamine receptor cDNA},
  author={James R. Bunzow and Hubert H. M. Van Tol and David K. Grandy and Paul R. Albert and John A. Salon and Macdonald J. Christie and Curtis A. Machida and Kim A. Neve and Olivier Civelli},
  journal={Nature},
  year={1988},
  volume={336},
  pages={783-787}
}
Dopamine receptors are classified into D1 and D2 subtypes on the basis of their pharmacological and biochemical characteristics1,2. The D2 dopamine receptor has been implicated in the pathophysiology and treatment of movement disorders3, schizophrenia4 and drug addiction5. The D2 dopamine receptor interacts with guanine nucleotide-binding proteins to induce second messenger systems6,7. Other members of the family of receptors that are coupled to G proteins share a significant similarity in… 
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References

SHOWING 1-10 OF 27 REFERENCES

An intronless gene encoding a potential member of the family of receptors coupled to guanine nucleotide regulatory proteins

A DNA fragment in the human genome is cloned and sequenced which cross-hybridizes with a full-length β2-adrenergic receptor probe at reduced stringency and appears to be intronless, containing an uninterrupted long open reading frame which encodes a putative protein with all the expected structural features of a G-protein-coupled receptor.

Cloning of the gene and cDNA for mammalian β-adrenergic receptor and homology with rhodopsin

Cloning of the gene and cDNA for the mammalian β2AR indicates significant amino-acid homology with bovine rhodopin and suggests that, like rhodopsin7, βAR possesses multiple membrane-spanning regions.

The classification of dopamine receptors: relationship to radioligand binding.

Since 1975, the elegantly simple radioligand binding technique has direct examination of the interactions of agonists and antagonists with putative dopamine receptors (DARs), and these studies form the major focus of this review.

Cloning, sequencing, and expression of the gene coding for the human platelet alpha 2-adrenergic receptor.

The gene for the human platelet alpha 2-adrenergic receptor has been cloned with oligonucleotides corresponding to the partial amino acid sequence of the purified receptor, and two related genes have been identified by low stringency Southern blot analysis.

cDNA eloping of bovine substance-K receptor through oocyte expression system

The result provides the first indication that the neuropeptide receptor has sequence similarity with rhodopsin-type receptors (the G-protein-coupled receptor family) and thus possesses multiple membrane-spanning domains.

Glycoprotein nature of D2 dopamine receptors

The D2-dopamine receptor of anterior pituitary is functionally associated with a pertussis toxin-sensitive guanine nucleotide binding protein.

Removal of phosphorylation sites from the β2-adrenergic receptor delays onset of agonist-promoted desensitization

It is reported that prevention of agonist-stimulated β2-adrenergic receptor (β2AR) phosphorylation by truncation of its serine and threonine-rich phosphate acceptor segment delays the onset of desensitization.

Quantitative autoradiographic localization of the D1 and D2 subtypes of dopamine receptors in rat brain

The distribution of D1 and D2 receptors was studied in coronal sections of rat brain, using quantitative autoradiography. D1 receptors were labeled with 1.8 nM 3H-SKF-83566 (a brominated analog of…

Expression of cDNAs for two isoforms of the catalytic subunit of cAMP-dependent protein kinase.