Cloning and expression of a new rat procarboxypeptidase B gene in Escherichia coli and purification of recombination carboxypeptidase B.

@article{Suxia2003CloningAE,
  title={Cloning and expression of a new rat procarboxypeptidase B gene in Escherichia coli and purification of recombination carboxypeptidase B.},
  author={Liu Su-xia and Zhang Yu-jian and Tian Li-ping and Yuan Qin-sheng and Gong Yi},
  journal={Protein and peptide letters},
  year={2003},
  volume={10 6},
  pages={
          581-90
        }
}
A new coding sequence of the procarboxypeptidase B gene was obtained from SD rat fresh pancreas by RT-PCR and highly expressed in Escherichia coli in inclusion bodies. The folded procarboxypeptidase B was subjected to trypsin enzymatic cleavage to produce active carboxypeptidase B, subsequently, carboxypeptidase B was effectively purified with anion exchange chromatography DEAE-FF and hydrophobic interaction chromatography Octyl FF, as a result, 40 mg carboxypeptidase B per litre cell culture… Expand
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