Cloning and expression of a human large-conductance calcium-activated potassium channel.

@article{Dworetzky1994CloningAE,
  title={Cloning and expression of a human large-conductance calcium-activated potassium channel.},
  author={Steven I. Dworetzky and Joanne T. Trojnacki and Valentin K. Gribkoff},
  journal={Brain research. Molecular brain research},
  year={1994},
  volume={27 1},
  pages={189-93}
}
A putative BK channel gene was cloned from a human brain (substantia nigra) cDNA library by hybridization screening. The sequence of the full length clone shows high homology with the mSlo gene, suggesting that this cDNA is the human homologue (hSlo). The hSlo clone does not contain either alternative exon A or B at its splice sites; and similar to mSlo, it has a long string of serines at its 5' end. Reverse transcription coupled with the PCR technique demonstrated the differences in expression… CONTINUE READING