Cloning and expression of a complementary DNA encoding a high affinity human neurotensin receptor

  title={Cloning and expression of a complementary DNA encoding a high affinity human neurotensin receptor},
  author={Natalio Vita and Patrick Laurent and Sylvie Lefort and Pascale Chalon and Xavier Dumont and Mourad Kaghad and Dani{\`e}le Gully and Gérard Le Fur and Pascual Ferrara and Daniel Caput},
  journal={FEBS Letters},

Cloning and characterization of the rat neurotensin receptor gene promoter.

Chromosomal localization of mouse and human neurotensin receptor genes

The present data demonstrate that the Ntsr gene is assigned to the H region of the mouse Chromosome (Chr) 2 and to the long arm of the human Chr 20.

The 100-kDa Neurotensin Receptor Is gp95/Sortilin, A Non-G-Protein-coupled Receptor*

Affinity labeling and binding experiments showed that the 110-kDa NT3 receptor can be partly transformed into a higher affinity by cotransfection with furin, which is the first transmembrane neuropeptide receptor that does not belong to the superfamily of G-protein-coupled receptors.

Molecular characterization of structure and tissue distribution of chicken neurotensin receptor.

Characterization of an antibody Fv fragment that binds to the human, but not to the rat neurotensin receptor NTS-1.

The Fv B-N6 fragment will be used to isolate a high-affinity binder to the human neurotensin receptor as a valuable tool for cocrystallization and receptor structure determination.

Transcriptional Regulation of the Tyrosine Hydroxylase Gene by Neurotensin in Human Neuroblastoma CHP212 Cells

Results indicate that modulation of tyrosine hydroxylase gene expression may constitute one of the mechanisms involved in the control of dopamine transmission by neurotensin and participate in multiple adaptation processes within the central nervous system to environmental conditions where neurotens in is released.



Purification of the neurotensin receptor from bovine brain.

Identification of a common nucleotide sequence in the 3'-untranslated region of mRNA molecules specifying inflammatory mediators.

A consensus sequence, comprised entirely of A and T residues and located in the 3'-untranslated region, is conserved in toto in the murine and human TNF mRNAs, suggesting that it may serve a specific regulatory function among the m RNAs in which it is found.

Neurotensin binding to extraneural and neural receptors: comparison with biological activity and structure--activity relationships.

A highly significant correlation was found between the two binding systems and the biological potencies obtained from the peptide abilities to contract isolated longitudinal smooth muscle strips of the guinea pig ileum when the binding affinities of neurotens in and neurotensin analogs were compared in the extraneural and neural systems.

Molecular cloning of the CD2 antigen, the T-cell erythrocyte receptor, by a rapid immunoselection procedure.

  • B. SeedA. Aruffo
  • Biology, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 1987
A cDNA encoding the CD2 antigen has been isolated by a highly efficient technique based on transient expression in COS cells and adherence of cells expressing surface antigen to antibody-coated

Characterization of Neurotensin Binding Sites in Intact and Solubilized Bovine Brain Membranes

Analysis of the equilibrium binding of [3H]‐neurotensin(1–13) at 25°C to its receptor sites in bovine cortex membranes indicated a single population of sites with an apparent equilibrium dissociation

Regulation of cyclic GMP levels by neurotensin in neuroblastoma clone N1E115.

Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

  • H. AvivP. Leder
  • Biology, Chemistry
    Proceedings of the National Academy of Sciences of the United States of America
  • 1972
A convenient technique for the partial purification of large quantities of functional, poly(adenylic acid)-rich mRNA is described and should prove generally useful as an initial step in the isolation of specific mRNAs.