Cloning and expression of a chick liver glutathione S-transferase CL 3 subunit with the use of a baculovirus expression system.

@article{Chang1992CloningAE,
  title={Cloning and expression of a chick liver glutathione S-transferase CL 3 subunit with the use of a baculovirus expression system.},
  author={Lindsay Chang and Jackie C. Y. Fan and L F Liu and S P Tsai and Ming F. Tam},
  journal={The Biochemical journal},
  year={1992},
  volume={281 ( Pt 2)},
  pages={545-51}
}
Glutathione S-transferase CL 3 subunits purified from 1-day-old-chick livers were digested with Achromobacter proteinase I and the resulting fragments were isolated for amino acid sequence analysis. An oligonucleotide probe was constructed accordingly for cDNA library screening. A cDNA clone of 1342 bases, pGCL301, encoding a protein of 26209 Da was isolated and sequenced. Including conservative substitutions, this protein has 75-79% sequence similarity to other Alpha family glutathione S… CONTINUE READING
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