Cloning and expression of a cDNA for the rat kappa-opioid receptor.

  title={Cloning and expression of a cDNA for the rat kappa-opioid receptor.},
  author={Masaru Minami and Takashi Toya and Y Katao and Keiko Maekawa and Shun Nakamura and T Onogi and Shuji Kaneko and Masamichi Satoh},
  journal={FEBS letters},
  volume={329 3},
We cloned a cDNA for the rat kappa-opioid receptor from a rat thalamus cDNA library. The deduced amino acid sequence consists of 380 residues with features shared by members of the G protein-coupled receptor family. The specific binding of [3H]bremazocine to the membrane of COS-7 cells transfected with the cDNA was displaced by kappa-specific opioid ligands, but not by mu- and delta-specific ligands. Xenopus oocytes injected with the in vitro transcribed mRNA responded to opioid ligands with… CONTINUE READING

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