Cloning and expression of a cDNA encoding uridine kinase from mouse brain.

  title={Cloning and expression of a cDNA encoding uridine kinase from mouse brain.},
  author={P. A. Ropp and T. Traut},
  journal={Archives of biochemistry and biophysics},
  volume={336 1},
  • P. A. Ropp, T. Traut
  • Published 1996
  • Biology, Medicine
  • Archives of biochemistry and biophysics
Uridine kinase is the rate-limiting enzyme in the pyrimidine salvage pathway of all mammalian cells. A cDNA for uridine kinase from mouse brain has been isolated, sequenced, and characterized. This is the first report of a complete nucleotide sequence for mammalian uridine kinase. The isolated cDNA is only 95% complete, missing the first 17 codons. The correct 5'-terminus sequence was obtained from high-stringency screening of a mouse liver genomic DNA library. The translated cDNA sequence… Expand
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