Cloning and expression of a cDNA encoding a novel human neurotrophic factor

  title={Cloning and expression of a cDNA encoding a novel human neurotrophic factor},
  author={Yoshihiko Kaisho and Koji Yoshimura and Kazuo Nakahama},
  journal={FEBS Letters},
Brain‐Derived Neurotrophic Factor Is More Highly Conserved in Structure and Function than Nerve Growth Factor During Vertebrate Evolution
The DNA‐deduced amino acid sequences of processed mature fish NGF and BDNF showed, compared to mouse, 63% and 90% identity, respectively, indicating that NGF had reached an optimized structure later than BDNF.
Production and characterization of recombinant mouse neurotrophin-3.
Neurotrophin-3 (NT-3) is a neurotrophic-factor that has recently been cloned on the basis of its structural similarity to other members of the nerve growth factor (NGF) gene family. In order to
Purification and characterization of biologically active recombinant human neurotrophin-3 produced by expression of a chimera gene in Chinese hamster ovary cells
The purified NT-3, at a concentration as low as 0.2 ng/ml, induced neurite out-growth in neurons prepared from 8-day-old chick embryonic dorsal root ganglia; however, it showed little neurotrophic effect on rat PC12 pheochromocytoma cells, which are known to be NGF-responding cells.
Brain-Derived Neurotrophic Factor ls More Highly Conserved in Structure and Function than Nerve Growth Factor During Vertebrate Evolution
The retrograde extrapolation of these data indicates that NGF and BDNF evolved at strikingly different rates ftom a common ancestral gene about 600 million years ago and reached an optimized structure later than BDNF.
Cellular Localization of Brain‐derived Neurotrophic Factor and Neurotrophin‐3 mRNA Expression in the Early Chicken Embryo
The regional expression pattern is in agreement with the established role for BDNF and NT‐3 as target‐derived neurotrophic factors, but the results also suggest that BDNF may be an intrinsic factor important for the development of the inner ear.
Production and Characterization of Recombinant Rat Brain‐Derived Neurotrophic Factor and Neurotrophin‐3 from Insect Cells
Pure, recombinant rat BDNF and NT‐3 promoted the survival of embryonic dorsal root ganglion neurons in the low picomolar range and are suitable for evaluation in animal models.


Neurotrophin-3: a neurotrophic factor related to NGF and BDNF.
The distribution of NT-3 messenger RNA and its biological activity on a variety of neuronal populations clearly distinguishNT-3 from NGF and BDNF, and provide compelling evidence that NT- 3 is an authentic neurotrophic factor that has its own characteristic role in vivo.
Purification of a new neurotrophic factor from mammalian brain.
This factor is the first neurotrophic factor to be purified since NGF, from which it is clearly distinguished because it has different antigenic and functional properties.
Development and regional expression of beta nerve growth factor messenger RNA and protein in the rat central nervous system.
Investigation of nerve growth factor (NGF) mRNA and protein in the rat central nervous system revealed a widespread distribution of NGF-like reactivity in the adult brain, preferentially in fiber tracts.
Human β-nerve growth factor gene sequence highly homologous to that of mouse
The isolation of mouse β-NGF complementary DNA (cDNA) is described and its nucleotide sequence is presented, which predicts a prepro-β- NGF molecule of Mr 27,000 (27K) and a pro-β -NGF molecules of Mr 25K.
Molecular cloning and expression of brain-derived neurotrophic factor
The full primary structure of brain-derived neurotrophic factor is reported and it is established that these two neurotrophic factors are related both functionally and structurally.
Expression and properties of two types of protein kinase C: alternative splicing from a single gene.
Two complementary DNA's, encoding the complete sequences of 671 and 673 amino acids for subspecies of rat brain protein kinase C, were expressed in COS 7 cells and showed practically identical physical and kinetic properties.
Expression of the beta-nerve growth factor gene correlates with the density of sympathetic innervation in effector organs.
  • D. Shelton, L. Reichardt
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1984
Levels of NGF mRNA were found that are too high to be attributed entirely to the vasculature, suggesting a role for NGF in adult central nervous system function.
The response of chick sensory neurons to brain-derived neurotrophic factor
The influence of BDNF and NGF on the survival and growth of the placode-derived and the neural crest-derived neurons of the trigeminal ganglion in dissociated neuron-enriched culture was studied.
Relationship between levels of nerve growth factor (NGF) and its messenger RNA in sympathetic ganglia and peripheral target tissues.
A sensitive assay for the quantification of nerve growth factor mRNA in various tissues of the mouse using in vitro transcribed RNANGF revealed that vas deferens contained high levels of both NGF and mRNANGF, whereas skeletal muscle levels were barely detectable, indicating that in peripheral tissues NGF levels are primarily regulated by the quantity of mRNangF.