Cloning and expression of a cDNA encoding a bovine brain brefeldin A-sensitive guanine nucleotide-exchange protein for ADP-ribosylation factor.

@article{Morinaga1997CloningAE,
  title={Cloning and expression of a cDNA encoding a bovine brain brefeldin A-sensitive guanine nucleotide-exchange protein for ADP-ribosylation factor.},
  author={Naoko Morinaga and Joel Moss and Martha Vaughan},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1997},
  volume={94 24},
  pages={12926-31}
}
A 200-kDa guanine nucleotide-exchange protein (p200 or GEP) for ADP-ribosylation factors 1 and 3 (ARF1 and ARF3) that was inhibited by brefeldin A (BFA) was purified earlier from cytosol of bovine brain cortex. Amino acid sequences of four tryptic peptides were 47% identical to that of Sec7 from Saccharomyces cerevisiae, which is involved in vesicular trafficking in the Golgi. By using a PCR-based procedure with two degenerate primers representing sequences of these peptides, a product similar… CONTINUE READING

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Morinaga et al

  • W. Kolanus, W. Nagel, +6 authors 233–242. Biochemistry
  • Proc. Natl. Acad. Sci. USA 94
  • 1997
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