Cloning and expression of a Paecilomyces thermophila xylanase gene in E. coli and characterization of the recombinant xylanase.

@article{Zhang2010CloningAE,
  title={Cloning and expression of a Paecilomyces thermophila xylanase gene in E. coli and characterization of the recombinant xylanase.},
  author={Min Zhang and Zheng-qiang Jiang and Shaoqing Yang and Chengwei Hua and Lite Li},
  journal={Bioresource technology},
  year={2010},
  volume={101 2},
  pages={688-95}
}
A cDNA library of Paecilomyces thermophila was constructed, and the gene encoding xylanase (designated Pt xynA) was isolated from the library. Pt xynA consisted of 681 bp, and the translated protein encoded 226 amino acids. This is the first functional gene cloned from P. thermophila. The gene was successfully expressed in Escherichia coli BL21 and the recombinant xylanase (XynA) was purified to homogeneity by Ni-NTA and Sephadex G50. XynA showed an optimum activity at 75 degrees C and pH 7.0… CONTINUE READING

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