Cloning and expression of R-factor mediated arsenate resistance in Escherichia coli

@article{Mobley1983CloningAE,
  title={Cloning and expression of R-factor mediated arsenate resistance in Escherichia coli},
  author={Harry L. T. Mobley and Chih-Ming Chen and Simon Silver and Barry P. Rosen},
  journal={Molecular and General Genetics MGG},
  year={1983},
  volume={191},
  pages={421-426}
}
The resistance transfer factor R773 confers inducible arsenate, arsenite and antimony resistance on Escherichia coli. The genes for these resistances were cloned into the EcoRi site of plasmid pBR322 to produce a 33 kilobase plasmid, pUM1. Bacterial strains transformed with pUM1 synthesized a polypeptide of the apparent molecular weight 64,000 daltons when induced with arsenite. This polypeptide could be visualized on sodium dodecyl sulfate polyacrylamide gels stained with Coomassie blue. It… CONTINUE READING

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Experiments in molecular genetics

  • J Miller
  • Cold Spring Harbor Laboratory Cold Spring Harbor,
  • 1972
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