Cloning and expression of DNA sequences associated with the killer trait of Paramecium tetraurelia stock 47.

@article{Quackenbush1983CloningAE,
  title={Cloning and expression of DNA sequences associated with the killer trait of Paramecium tetraurelia stock 47.},
  author={R L Quackenbush and James A. Burbach},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1983},
  volume={80 1},
  pages={250-4}
}
We have presented direct evidence that at least one of the traits associated with killing of paramecia by kappa particles is determined by an extrachromosomal genetic element. Plasmid DNA was isolated from Caedibacter taeniospiralis 47 (commonly known as 47 kappa), which is an obligate cytoplasmic endosymbiont of Paramecium tetraurelia. Fragments of pKAP47 DNA generated by Pst I digestion were inserted into pBR328 and then introduced into Escherichia coli 294 by transformation. Clones carrying… CONTINUE READING

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