Cloning and expression of Candida guilliermondii xylose reductase gene (xyl1) in Pichia pastoris

  title={Cloning and expression of Candida guilliermondii xylose reductase gene (xyl1) in Pichia pastoris},
  author={Chakkrapong Handumrongkul and D.-P. Ma and Juan L. Silva},
  journal={Applied Microbiology and Biotechnology},
A xylose reductase gene (xyl1) of Candida guilliermondii ATCC 20118 was cloned and characterized. The open reading frame of xyl1 contained 954 nucleotides encoding a protein of 317 amino acids with a predicted molecular mass of 36 kDa. The derived amino acid sequence of C. guilliermondii xylose reductase was 70.4% homologous to that of Pichia stipitis. The gene was placed under the control of an alcohol oxidase promoter (AOX1) and integrated into the genome of a methylotrophic yeast, Pichia… CONTINUE READING


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