Cloning and expression of β-galactosidase from psychotrophic Bacillus subtilis KL88 into Escherichia coli

@article{Torres1995CloningAE,
  title={Cloning and expression of β-galactosidase from psychotrophic Bacillus subtilis KL88 into Escherichia coli},
  author={Mar{\'i}a J. Torres and Byong Hoon Lee},
  journal={Biotechnology Letters},
  year={1995},
  volume={17},
  pages={123-128}
}
SummaryThe β-galactosidase gene from Bacillus subtilis KL88 was cloned into Escherichia coli and the gene product characterized for its potential use in the dairy industry. The two recombinant plasmids that we obtanied encoded a β-galactosidase with the same catalytic and thermal characteristics as the native β-galactosidase from B. subtilis. The recombinant β-galactosidases exhibited high activity at low temperature (10°C), with maximum activity at 50°C and an optimum pH of 6.0. Its molecular… CONTINUE READING

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