Cloning and expression inEscherichia coli of the gene encoding β‐C‐4‐oxygenase, that converts β‐carotene to the ketocarotenoid canthaxanthin inHaematococcus pluvialis

@article{Lotan1995CloningAE,
  title={Cloning and expression inEscherichia coli of the gene encoding $\beta$‐C‐4‐oxygenase, that converts $\beta$‐carotene to the ketocarotenoid canthaxanthin inHaematococcus pluvialis},
  author={T. Lotan and J. Hirschberg},
  journal={FEBS Letters},
  year={1995},
  volume={364}
}
In the green algaHaematococcus pluvialis the ketocarotenoid astaxanthin accumulates under stress conditions. Astaxanthin is a red carotenoid pigment which possess antioxidative activity. We have cloned the gene for β‐C‐4 oxygenase (β‐carotene ketolase) from the green algaeH. pluvialis. The cloning method took advantage of a strain ofE. coli which was genetically engineered to produce β‐carotene. An expression cDNA library ofH. pluvialis was transfected to cells of this strain and visually… Expand
Expression in Escherichia coli and properties of the carotene ketolase from Haematococcus pluvialis
TLDR
It is concluded that the carotenoids zeaxanthin and adonixanthin which accumulate in addition to astaxanthIn in this transformant are products of side reactions rather than direct precursors of astaxantin. Expand
Regulation of astaxanthin and its intermediates through cloning and genetic transformation of β-carotene ketolase in Haematococcus pluvialis.
TLDR
The expression level of carotenogenic genes like phy toene synthase (psy), phytoene desaturase (pds), lycopene cyclase (lcy), bkt, and β-carotene hydroxylase (bkh) were found to be higher in transformed cells compared to the non-transformed H. pluvialis. Expand
Isolation and characterization of a carotenoid oxygenase gene from Chlorella zofingiensis (Chlorophyta)
TLDR
Functional complementation in Escherichia coli showed that the coding protein of the crtO gene not only exhibits normal CRTO activity by converting β-carotene to canthaxanthin via echinenone, but also displays a high enzymatic activity of converting zeaxanthIn to astaxanth in via adonixanthin. Expand
Isolation of the β-carotene ketolase gene promoter from Haematococcus pluvialis and expression of ble in transgenic Chlamydomonas
TLDR
It is confirmed that the bkt1 promoter owned cis-acting elements involved in light and environmental stresses and the genetic transformation system of C. reinhardtii can be used to study the functions of bkt 1promoters from H. pluvialis. Expand
Erratum to: Cloning and selection of carotenoid ketolase genes for the engineering of high-yield astaxanthin in plants
β-Carotene ketolase (BKT) catalyzes the rate-limiting steps for the biosynthesis of astaxanthin. Several bkt genes have been isolated and explored to modify plant carotenoids to astaxanthin withExpand
Title Isolation and characterization of a carotenoid oxygenase genefrom
The green alga Chlorella zofingiensis produces large amounts of the valuable ketocarotenoid astaxanthin under dark-heterotrophic growth conditions, making it potentially employable for commercialExpand
Turning a green alga red: engineering astaxanthin biosynthesis by intragenic pseudogene revival in Chlamydomonas reinhardtii
TLDR
Engineered C. reinhardtii strains could be a promising alternative to natural astaxanthin producing algal strains and may open the possibility of other tailor‐made pigments from this host. Expand
Conversion of β-carotene into astaxanthin: Two separate enzymes or a bifunctional hydroxylase-ketolase protein?
TLDR
A bifunctional β-carotene hydroxylase-ketolase activity has been proposed for the CrtS protein, and the evidence for and against this hypothesis is analyzed in detail in this review. Expand
Metabolic engineering of ketocarotenoids biosynthesis in the unicelullar microalga Chlamydomonas reinhardtii.
TLDR
In this study the unicellular chlorophyte Chlamydomonas reinhardtii has been genetically engineered with the beta-carotene ketolase cDNA from Haematococcus pluvialis to obtain a transgenic microalga able to synthesize ketocarotenoids. Expand
Metabolic engineering of ketocarotenoids biosynthesis in the unicelullar microalga Chlamydomonas reinhardtii
TLDR
In this study the unicellular chlorophyte Chlamydomonas reinhardtii has been genetically engineered with the -carotene ketolase cDNA from Haematococcus pluvialis to obtain a transgenic microalga able to synthesize ketocarotenoids. Expand
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