Cloning and expression in Escherichia coli of the cDNA for murine tumor necrosis factor.

  title={Cloning and expression in Escherichia coli of the cDNA for murine tumor necrosis factor.},
  author={Diane Pennica and Joel S. Hayflick and Timothy S. Bringman and Michael A Palladino and David V. Goeddel},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={82 18},
A murine tumor necrosis factor (MuTNF) cDNA was isolated from a cDNA library prepared by using mRNA from the murine macrophage-like cell line PU5-1.8 induced with 4 beta-phorbol 12 beta-myristate 13 alpha-acetate. The cDNA encodes a polypeptide consisting of a 79 amino acid pre sequence followed by a mature MuTNF sequence of 156 amino acids. The 235 amino acid murine pre-TNF polypeptide is 79% homologous to the human pre-TNF protein. There is one potential N-linked glycosylation site on MuTNF… CONTINUE READING

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