Cloning and disruption of a fragment of Streptomyces halstedii DNA involved in the biosynthesis of a spore pigment.

@article{Blanco1992CloningAD,
  title={Cloning and disruption of a fragment of Streptomyces halstedii DNA involved in the biosynthesis of a spore pigment.},
  author={Gloria Blanco and Ariel Pereda and Carmen M{\'e}ndez and Jos{\'e} A Salas},
  journal={Gene},
  year={1992},
  volume={112 1},
  pages={59-65}
}
A 5.2-kb BamHI fragment of Streptomyces halstedii was cloned by homology to the actI-carrying fragment which codes for part of the actinorhodin polyketide synthase of Streptomyces coelicolor A3(2). Gene disruption using the integrative plasmid vector, pGM160, and gene replacement experiments using a fragment mutated by introducing a cassette containing the gene encoding thiostrepton resistance, showed that the alteration of this region in the chromosome of S. halstedii caused sporulating… CONTINUE READING