Cloning and direct G-protein regulation of phospholipase D from tobacco.

  title={Cloning and direct G-protein regulation of phospholipase D from tobacco.},
  author={W Lein and Gerhard Saalbach},
  journal={Biochimica et biophysica acta},
  volume={1530 2-3},
Phospholipase D (PLD) and heterotrimeric G-proteins are involved in plant signal transduction pathways at the plasma membrane. There is evidence suggesting that PLD acts downstream from G-proteins, but a direct interaction of specific members has not been shown. In the present paper, a PLD cDNA clone was isolated from tobacco, expressed as a GST fusion in bacteria, and the recombinant protein was purified by glutathione affinity. Its enzymatic properties identified it as an alpha-type PLD. The… CONTINUE READING

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