Cloning and comparison of ten gene sequences of a Chilean H. pylori strain with other H. pylori strains revealed higher variability for VacA and CagA virulence factors.

@article{Mller2002CloningAC,
  title={Cloning and comparison of ten gene sequences of a Chilean H. pylori strain with other H. pylori strains revealed higher variability for VacA and CagA virulence factors.},
  author={Ilse M{\"u}ller and Angelica Medina-Selby and Jos{\'e} Luis Palacios and Patricio Martinez and Patricio Opazo and E. G. Bruce and Marta Mancilla and Pablo Valenzuela and Arturo Yudelevich and Alejandro Venegas},
  journal={Biological research},
  year={2002},
  volume={35 1},
  pages={67-84}
}
We have cloned and sequenced ten Helicobacter pylori genes from a Chilean strain (CH-CTX1) including: a cytotoxin VacA fragment, a CagA fragment (A17), a species-specific protein (TsaA), urease subunits (UreA, UreB), a flagellin subunit (FlaB), heat shock proteins (HspA and HspB), adhesin (HpaA) and a lipoprotein (Lpp20). We compared their deduced amino acid sequences with the corresponding sequences from three unrelated H. pylori strains, including fully sequenced strains 26695(UK) and J99(USA… CONTINUE READING