Cloning and chromosomal mapping of URA3 genes of Pichia farinosa and P. sorbitophila encoding orotidine-5'-phosphate decarboxylase.


The PfURA3 gene, which encodes orotidine-5'-phosphate decarboxylase, of osmotolerant yeast Pichia farinosa NFRI 3,621, was cloned by complementation of the ura3 mutation of Saccharomyces cerevisiae. The nucleotide sequence of the PfURA3 gene and its deduced amino acid sequence indicated that the gene encodes a protein (PfUra3p) of 267 amino acids. Pulsed… (More)