Cloning and characterization of two genes encoding dihydroxyacetone kinase from Schizosaccharomyces pombe IFO 0354.

@article{Kimura1998CloningAC,
  title={Cloning and characterization of two genes encoding dihydroxyacetone kinase from Schizosaccharomyces pombe IFO 0354.},
  author={Tetsuya Kimura and Miyako Takahashi and Kumiko Yoshihara and Takuya Furuichi and Katsuhisa Suzuki and Kohsuke Imai and Shuichi Karita and Kazuo Sakka and Kunio Ohmiya},
  journal={Biochimica et biophysica acta},
  year={1998},
  volume={1442 2-3},
  pages={361-8}
}
We report the cloning and characterization of two genes encoding dihydroxyacetone kinase (EC 2.7.1.29), SpDAK1 and SpDAK2, from Schizosaccharomyces pombe IFO 0354. The open reading frames of both genes encode 591 amino acids and have Mrs of 62158 and 62170, respectively. Both predicted amino acid sequences exhibited a high identity to each other (99.8%) and relatively high identities (30% to 76%) to other putative dihydroxyacetone kinase gene products. A Western blot analysis showed that these… CONTINUE READING