Cloning and characterization of two β-glucosidase/xylosidase enzymes from yak rumen metagenome.

@article{Bao2012CloningAC,
  title={Cloning and characterization of two β-glucosidase/xylosidase enzymes from yak rumen metagenome.},
  author={Lei Bao and Qiang Huang and Lei Chang and Qingwen Sun and Jungang Zhou and Hong Lu},
  journal={Applied biochemistry and biotechnology},
  year={2012},
  volume={166 1},
  pages={72-86}
}
Two β-glucosidase/xylosidase genes, Rubg3A and Rubg3B, were cloned from yak rumen uncultured microorganisms by metagenome method and function-based screening. Recombinant RuBG3A and RuBG3B purified from Escherichia coli were characterized for enzymatic properties, and they exhibited activity against 4-nitrophenyl-β-D: -glucopyranoside and 4-nitrophenyl-β-D: -xylopyranoside, suggesting bifunctional β-glucosidase/xylosidase activity. Chromatography analysis showed that they could effectively… CONTINUE READING

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