Cloning and characterization of two K+ inward rectifier (Kir) 1.1 potassium channel homologs from human kidney (Kir1.2 and Kir1.3).

@article{Shuck1997CloningAC,
  title={Cloning and characterization of two K+ inward rectifier (Kir) 1.1 potassium channel homologs from human kidney (Kir1.2 and Kir1.3).},
  author={Mary E. Shuck and Timothy M. Piser and Jeffrey H. Bock and Jerry L. Slightom and Kyunggeun Lee and Michael Jerome Bienkowski},
  journal={The Journal of biological chemistry},
  year={1997},
  volume={272 1},
  pages={
          586-93
        }
}
The DNA sequence encoding the rat brain inward rectifier-10 K+ channel was amplified from rat brain RNA using reverse transcription-polymerase chain reaction and used to clone the human homolog. Low stringency screening of a human kidney cDNA library and subsequent DNA sequence analysis identified two related K+ inward rectifier cDNAs, referred to as Kir1.2 and Kir1.3, which were derived from transcription of distinct human genes. Kir1.2 represents the human homolog of the rat BIRK-10 sequence… CONTINUE READING

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