Cloning and characterization of the yeast CKI gene encoding choline kinase and its expression in Escherichia coli.

@article{Hosaka1989CloningAC,
  title={Cloning and characterization of the yeast CKI gene encoding choline kinase and its expression in Escherichia coli.},
  author={Kaoru Hosaka and Tsutomu Kodaki and Shinya Yamashita},
  journal={The Journal of biological chemistry},
  year={1989},
  volume={264 4},
  pages={2053-9}
}
Using a mutant defective in choline kinase (Hosaka, K., and Yamashita, S. (1980) J. Bacteriol. 143, 176-181; Hosaka, K., and Yamashita, S. (1987) Eur. J. Biochem. 162, 7-13), the structural gene (CKI) for choline kinase of the yeast, Saccharomyces cerevisiae, was isolated by means of genetic complementation. Within its sequence there was an open reading… CONTINUE READING