Cloning and characterization of the structural gene for the class 2 protein of Neisseria meningitidis.

@article{Murakami1989CloningAC,
  title={Cloning and characterization of the structural gene for the class 2 protein of Neisseria meningitidis.},
  author={Katsuhiko S Murakami and Emil C. Gotschlich and Michael Seiff},
  journal={Infection and immunity},
  year={1989},
  volume={57 8},
  pages={2318-23}
}
The class 2 protein of Neisseria meningitidis is the major outer membrane protein and a porin. A lambda gt11 bank of meningococcal chromosomal DNA was screened with monoclonal antibodies against gonococcal protein IB that cross-react with meningococcal class 2 protein. Three independent immunoreactive clones were isolated. DNA sequence analysis indicated that these clones included regions encoding the N-terminal portion of the class 2 protein. An oligonucleotide of 21 bases that was… CONTINUE READING

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