Cloning and characterization of the ori region of pSW1200 of Erwinia stewartii: similarity with plasmid P1.

@article{Fu1997CloningAC,
  title={Cloning and characterization of the ori region of pSW1200 of Erwinia stewartii: similarity with plasmid P1.},
  author={Jun Feng Fu and Shuqin Ying and Shui Teng Liu},
  journal={Plasmid},
  year={1997},
  volume={38 3},
  pages={141-7}
}
The ori region of an Erwinia stewartii plasmid, pSW1200 (106 kb), has been cloned and sequenced. This region consists of a gene encoding a protein which has 91% similarity and 73% identity with the RepA protein of bacteriophage P1. The ori region also consists of eight copies of 19-bp iterons which are highly homologous to the iterons of P1. Similar to plasmid P1, pSW1200 replicon has a copy number of approximately 1. On the other hand, the copy number increases about ninefold if three of the… CONTINUE READING