Cloning and characterization of the natural lactose operator.

  title={Cloning and characterization of the natural lactose operator.},
  author={Jane Sadler and M L Tecklenburg},
  volume={13 1},
A 55-bp DNA segment carrying the wild-type lactose operator sequence has been cloned. Its sequence is: (Formula: see text). With the exceptions of the bases at positions 19 and 41, 26 and 34, and 28 and 32, the sequence is a perfect inverted repeat about base pair 30. This segment was obtained from the wild-type lactose promoter and operator region of lambda h80dlac phage DNA by a combination of in vitro and in vivo steps. Up to four direct-repeat copies of this segment have been cloned in… CONTINUE READING

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