Cloning and characterization of the gene encoding a repressible acid phosphatase (PHO1) from the methylotrophic yeast Hansenula polymorpha

@article{Phongdara1998CloningAC,
  title={Cloning and characterization of the gene encoding a repressible acid phosphatase (PHO1) from the methylotrophic yeast Hansenula polymorpha},
  author={Amornrat Phongdara and Armin Merckelbach and Peter Keup and Gerd Gellissen and Cornelis P. Hollenberg},
  journal={Applied Microbiology and Biotechnology},
  year={1998},
  volume={50},
  pages={77-84}
}
A cloned cDNA, generated from mRNA isolates of phosphate-derepressed H. polymorpha cells, was identified to harbour an incomplete sequence of the coding region for a repressible acid phosphatase. The cDNA fragment served as a probe to screen a plasmid library of H. polymorpha genomic DNA. A particular clone, p606, of a 1.9-kb insert contained a complete copy of the PHO1 gene. Sequencing revealed the presence of a 1329-nucleotide open reading frame encoding a protein of 442 amino acids with a… CONTINUE READING
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Cloning and sequencing of the ura3 locus of the methylotrophic yeast Hansenula polymorpha and its use for the generation of a deletion by gene replacement

  • A Merckelbach, S GoÈ decke, ZA Janowicz, CP Hollenberg
  • Appl Microbiol Biotechnol
  • 1993
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