Cloning and characterization of the chromosomal replication origin region of Amycolatopsis mediterranei U32.

@article{Tian2005CloningAC,
  title={Cloning and characterization of the chromosomal replication origin region of Amycolatopsis mediterranei U32.},
  author={Yongqiang Tian and Pei Hao and Guo-Ping Zhao and Zhongjun Qin},
  journal={Biochemical and biophysical research communications},
  year={2005},
  volume={333 1},
  pages={14-20}
}
The chromosomal replication origins (oriC) of gram positive, acid-fast actinomycetes have been investigated in streptomycetes and mycobacteria. A 1339 bp DNA fragment of the putative oriC region from the rifamycin SV producer Amycolatopsis mediterranei U32 was cloned by PCR amplification employing primers designed based on the conserved flanking genes of… CONTINUE READING