Cloning and characterization of the Neisseria meningitidis rfaC gene encoding alpha-1,5 heptosyltransferase I.

@article{Stojiljkovi1997CloningAC,
  title={Cloning and characterization of the Neisseria meningitidis rfaC gene encoding alpha-1,5 heptosyltransferase I.},
  author={Igor Stojiljkovi{\'c} and Vivian Hwa and Jennifer Larson and L F Lin and Magdalene So and Xavier Nassif},
  journal={FEMS microbiology letters},
  year={1997},
  volume={151 1},
  pages={41-9}
}
We cloned and characterized the Neisseria meningitidis rfaC gene which encodes an enzyme, alpha-1,5 heptosyltransferase I, involved in the synthesis of the deep-core of the lipooligosaccharide. The N. meningitidis rfaC mutant, obtained by an allelic exchange, produced lipooligosaccharide which migrated faster in sodium dodecyl sulfate-polyacrylamide gel electrophoresis than the lipooligosaccharide isolated from the wild-type N. meningitidis. The N. meningitidis rfaC mutant was not affected by… CONTINUE READING