Cloning and characterization of the Flavobacterium johnsoniae (Cytophaga johnsonae) gliding motility gene, gldA.

@article{Agarwal1997CloningAC,
  title={Cloning and characterization of the Flavobacterium johnsoniae (Cytophaga johnsonae) gliding motility gene, gldA.},
  author={Sadhana Agarwal and David Hunnicutt and Mark J McBride},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1997},
  volume={94 22},
  pages={12139-44}
}
The mechanism of bacterial gliding motility (active movement over surfaces without the aid of flagella) is not known. A large number of nonmotile mutants of the gliding bacterium Flavobacterium johnsoniae (Cytophaga johnsonae) have been previously isolated, and genetic techniques to analyze these mutants have recently been developed. We complemented a nonmotile mutant of F. johnsoniae (UW102-09) with a library of wild-type DNA by using the shuttle cosmid pCP17. The complementing plasmid (pCP100… CONTINUE READING