Cloning and characterization of the Chlamydomonas moewusii mitochondrial genome

@article{Lee1991CloningAC,
  title={Cloning and characterization of the Chlamydomonas moewusii mitochondrial genome},
  author={Robert W. Lee and Carole Dumas and Claude Lemieux and Monique Turmel},
  journal={Molecular and General Genetics MGG},
  year={1991},
  volume={231},
  pages={53-58}
}
We report that the mitochondrial genome of Chlamydomonas moewusii has a 22 kb circular map and thus contrasts with the mitochondrial genome of Chlamydomonas reinhardtii, which is linear and about 6 kb shorter. Overlapping restriction fragments spanning over 90% of the C. moewusii mitochondrial DNA (mtDNA) were identified in a clone bank constructed using a Sau3AI partial digest of a C. moewusii DNA fraction enriched for mtDNA by preparative CsCI density gradient centrifugation. Overlapping… CONTINUE READING

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