Cloning and characterization of the Bg/II restriction-modification system reveals a possible evolutionary footprint.

@article{Anton1997CloningAC,
  title={Cloning and characterization of the Bg/II restriction-modification system reveals a possible evolutionary footprint.},
  author={Brian P. Anton and Daniel F. Heiter and Jack S. Benner and Ellen J Hess and L Greenough and Larry Moran and Barton E. Slatko and Joan E. Brooks},
  journal={Gene},
  year={1997},
  volume={187 1},
  pages={19-27}
}
Bg/II, a type II restriction-modification (R-M) system from Bacillus globigii, recognizes the sequence 5'-AGATCT-3'. The system has been cloned into E. coli in multiple steps: first the methyltransferase (MTase) gene, bglIIM, was cloned from B. globigii RUB561, a variant containing an inactivated endonuclease (ENase) gene (bglIIR). Next the ENase protein (R.BglII) was purified to homogeneity from RUB562, a strain expressing the complete R-M system. Oligonucleotide probes specific for the 5' end… CONTINUE READING