Cloning and characterization of the ALG3 gene of Saccharomyces cerevisiae.

  title={Cloning and characterization of the ALG3 gene of Saccharomyces cerevisiae.},
  author={Markus Aebi and Johann Gassenhuber and Horst Domdey and S te Heesen},
  volume={6 4},
The Saccharomyces cerevisiae alg3-1 mutant is described as defective in the biosynthesis of dolichol-linked oligosaccharides (Huffaker and Robbins, Proc. Natl. Acad. Sci. USA, 80, 7466-7470, 1983). Man5GlcNAc2-PP-Dol accumulates in alg3 cells and Endo H resistant carbohydrates are transferred to protein by the oligosaccharyltransferase complex. In this study, we describe the cloning of the ALG3 locus by complementation of the temperature sensitive growth defect of the alg3 stt3 double mutant… CONTINUE READING


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