Cloning and characterization of pvdS, a gene required for pyoverdine synthesis in Pseudomonas aeruginosa: PvdS is probably an alternative sigma factor

@article{Cunliffe1995CloningAC,
  title={Cloning and characterization of pvdS, a gene required for pyoverdine synthesis in Pseudomonas aeruginosa: PvdS is probably an alternative sigma factor},
  author={Heather E. Cunliffe and Tony Raymond Merriman and Iain L. Lamont},
  journal={Journal of Bacteriology},
  year={1995},
  volume={177},
  pages={2744 - 2750}
}
Cells of Pseudomonas aeruginosa secrete a fluorescent yellow-green siderophore, pyoverdine, when grown under iron-deficient conditions. We describe here the cloning and characterization of a gene, pvdS, which is required for this process. The pvdS gene is required for expression from promoters of at least two pyoverdine synthesis genes and can cause expression from these promoters in Escherichia coli, where they are otherwise inactive. Sequencing of pvdS revealed that it is a member of a… Expand
Functional Analysis of PvdS, an Iron Starvation Sigma Factor of Pseudomonas aeruginosa
TLDR
Evidence is provided that the PvdS protein of P. aeruginosa is endowed with biochemical properties of eubacterial sigma factors, as it spontaneously forms 1:1 complexes with the core fraction of RNA polymerase (RNAP, alpha(2)betabeta' subunits), thereby promoting in vitro binding of the PvDS-RNAP holoenzyme to the promoter region of the pvdA gene. Expand
Transcriptional control of the pvdS iron starvation sigma factor gene by the master regulator of sulfur metabolism CysB in Pseudomonas aeruginosa.
TLDR
These findings establish a novel molecular link between the iron and sulfur regulons in P. aeruginosa, and identify and functional characterization of the LysR-like transcription factor CysB as a novel regulator of pvdS transcription. Expand
Iron-regulated transcription of the pvdA gene in Pseudomonas aeruginosa: effect of Fur and PvdS on promoter activity
TLDR
The results support the hypothesis that the P. aeruginosa Fur repressor indirectly controls pvdA transcription through the intermediary sigma factor PvdS; in the presence of sufficient iron, Fur blocks the pvdS promoter, thus preventing PvDS expression and consequently transcription of pVDA and other pyoverdin biosynthesis genes. Expand
A positive regulatory gene,pvdS, for expression of pyoverdin biosynthetic genes inPseudomonas aeruginosa PAO
TLDR
In response to iron limitationPseudomonas aeruginosa PAO induces production of pyoverdin, a low-molecular-weight siderophore able to capture ferric ion with a very high affinity, which shows considerable similarity to FecI of E. coli, a positive regulator for transcription of the ferric citrate transport system operon. Expand
Involvement of a transformylase enzyme in siderophore synthesis in Pseudomonas aeruginosa.
TLDR
The characterization of the pvdF gene, which is required for synthesis of the siderophore pyoverdine by Pseudomonas aeruginosa strain PAO1, is described, and the first example of involvement of a GART-type enzyme in synthesis of a secondary metabolite is provided. Expand
Regulation of the Pseudomonas aeruginosa toxA, regA and ptxR genes by the iron-starvation sigma factor PvdS under reduced levels of oxygen.
TLDR
It is proposed that the regulation of toxA expression under aerobic conditions primarily involves PvdS, while an additional EO-responsive regulator(s) besides Pvd S is required under low EO levels. Expand
Involvement of AlgQ in Transcriptional Regulation of Pyoverdine Genes in Pseudomonas aeruginosa PAO1
TLDR
It is proposed that AlgQ acts as an anti-sigma factor for RpoD, eliciting core RNA polymerase recruitment by PvdS and transcription initiation at pvd promoters, and provides a link between the pyoverdine and alginate regulatory networks. Expand
Characterization of the PvdS‐regulated promoter motif in Pseudomonas syringae pv. tomato DC3000 reveals regulon members and insights regarding PvdS function in other pseudomonads
TLDR
The role of PvdS as a regulator of pyoverdine synthesis is conserved among the fluorescent pseudomonads, but the promoters recognized by Pvd S orthologues may differ subtly from species to species. Expand
Characterisation of the pvdE gene which is required for pyoverdine synthesis in Pseudomonas aeruginosa.
TLDR
The deduced amino acid sequence of pvdE indicates that the protein is a member of the ATP-binding-cassette (ABC) family of membrane transporter proteins, and this is the first example of the involvement of an ABC-type protein in siderophore synthesis. Expand
Identification and characterization of novel pyoverdine synthesis genes in Pseudomonas aeruginosa.
TLDR
It is likely that most if not all of the genes required for pyoverdine synthesis in P. aeruginosa PAO1 have now been identified and this will form the basis for a biochemical description of the pathway of pyoverDine synthesis. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 45 REFERENCES
Identification and characterization of a siderophore regulatory gene (pfrA) of Pseudomonas putida WCS358: homology to the alginate regulatory gene algQ of Pseudomonas aeruginosa.
TLDR
It is concluded that PfrA is involved in the positive regulation of siderophore biosynthetic genes in response to iron limitation; furthermore, pfrA and algQ appeared to be interchangeable between P. putida and P. aeruginosa. Expand
Identification and characterization of a siderophore regulatory gene (pfrA) of Pseudomonas putida WCS358: homology to the alginate regulatory gene aigQ of Pseudomonas aeruginosa
TLDR
It is concluded that PfrA is involved in the positive regulation of siderophore biosynthetic genes in response to iron limitation; furthermore, pfrA and algQ appeared to be interchangeable between P. putida and P. aeruginosa. Expand
Coordinate regulation of siderophore and exotoxin A production: molecular cloning and sequencing of the Pseudomonas aeruginosa fur gene
TLDR
A positive selection procedure involving the isolation of manganese-resistant mutants was used to isolate mutants of strain PA103 that produce altered Fur proteins that constitutively produce siderophores and exotoxin A when grown in concentrations of iron that normally repress their production. Expand
Iron‐responsive gene expression in Pseudomonas fluorescens M114; cloning and characterization of a transcription‐activating factor, PbrA
TLDR
DNA sequence analysis of the complementing region identified one open reading frame (ORF) termed pbrA (pseuciobactin regulation activation) and Sequences upstream of the p brA gene suggest that transcription of pbrS may also be iron regulated, and domains with significant homology to a number of ECF transcriptional regulators of the σ70 sigma factor family. Expand
Iron regulation of siderophore biosynthesis and transport in Pseudomonas putida WCS358: involvement of a transcriptional activator and of the Fur protein
TLDR
The proposed role of Pfrl in strain WCS358 is an activator protein regulating pseudobactin 358 biosynthesis under iron limitation, which is recognized by Fur, an iron‐binding repressor protein found in many different bacteria. Expand
Nucleotide sequence of pvdD, a pyoverdine biosynthetic gene from Pseudomonas aeruginosa: PvdD has similarity to peptide synthetases
TLDR
A gene, pvdD, required for pyoverdine synthesis is sequenced, indicating that synthesis of the peptide moiety of py overdine proceeds by a nonribosomal mechanism. Expand
Cloning and sequence analysis of a gene (pchR) encoding an AraC family activator of pyochelin and ferripyochelin receptor synthesis in Pseudomonas aeruginosa
TLDR
A role for PchR as a transcriptional activator of pyochelin and ferripyochelin receptor synthesis in P. aeruginosa is consistent with a role for this mutant obtained by in vitro mutagenesis and gene replacement. Expand
Construction and use of a self-cloning promoter probe vector for gram-negative bacteria.
TLDR
The construction and use of a Tn5 derivative which contains the ColE1 origin of DNA replication, thereby allowing the cloning of DNA adjacent to the Tn without the need for construction of genomic libraries is described. Expand
Mapping of mutations affecting pyoverdine production in Pseudomonas aeruginosa
TLDR
At least 2 genes or gene clusters required for pyoverdine production in P. aeruginosa are identified and added to media containing EDDHA restored growth of pvd mutants. Expand
Operator sequences of the aerobactin operon of plasmid ColV-K30 binding the ferric uptake regulation (fur) repressor
The promoter region of the pColV-K30-encoded operon specifying biosynthesis and transport of the siderophore aerobactin was subjected to deletion analysis to determine the smallest DNA sequenceExpand
...
1
2
3
4
5
...