Cloning and characterization of five overlapping cDNAs specific for the human pro alpha 1(I) collagen chain.

@article{Chu1982CloningAC,
  title={Cloning and characterization of five overlapping cDNAs specific for the human pro alpha 1(I) collagen chain.},
  author={Mon Li Chu and Jeanne C. Myers and Michael P. Bernard and Junjia Ding and Francesco Ramirez},
  journal={Nucleic acids research},
  year={1982},
  volume={10 19},
  pages={
          5925-34
        }
}
We report the cloning of five overlapping cDNAs bearing sequences specific for the human pro alpha 1(I) collagen chain. Poly-A RNA enriched for collagen sequences was purified from normal human fibroblasts and used as template to synthesize double stranded cDNA. The cDNA was inserted into the Eco RI site of pBR 322 by blunt-ending and dG:dC tailing. The clones were screened by colony hybridization using the original RNA population and the resulting five positive clones subjected to restriction… CONTINUE READING
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